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Figure 1
Autophagic vesicle formation in the heart ventricle is increased during zebrafish cardiac regeneration. Electron microscopy shows a high degree of tissue remodeling of the cardiac muscle, as well as the formation of several autophagosome and phagolysosome vesicles upon apex amputation (yellow arrow heads and higher-magnification images below). An increase in the number of autophagic vesicles was above all evident in regions closer to the injured area (proximal), while also found significantly more abundant at 3 dpa distal to the amputation plane. Insets represent higher magnifications of the observed autophagic vesicles at each experimental time point. Scale bars represent 2 μm. *p ≤ 0.05. N ≥ 3.