ZFIN Image: Park et al., 2019, Fig. 2

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Figure Caption

Fig. 2

LREs Specifically Express a Multi-ligand Endocytic Machinery Composed of Cubn, Amn, and Dab2

(A) Isolation and analysis of gene expression profiles in LREs versus IEC. 6 dpf larvae expressing Cldn15la-GFP throughout the gut were gavaged with alexa-568 dextran (left panel) and subjected to FACS to isolate IECs and LREs for RNA-seq (right panel). Scale bar, 100 μm.

(B) Heatmap of RNA-seq data depicting expression levels of selected genes in LREs and IECs. Three independent samples for each cell population were used for RNA-seq experiment.

(C) Cartoon illustrating the multi-ligand endocytic machinery composed of Cubn, Amn, and Dab2.

(D) In situ hybridization of cubn (cubilin), amn (amnionless), dab2, and lrp2a (megalin). cubn, amn, and dab2 transcripts are enriched in LREs and pronephros. Bracket marks LRE region devoid of lrp2a transcript expression. PN = pronephros. n ≥ 20 animals, Scale bar, 100 μm. See also Figures S4 and S5.

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Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Cell, 51(1), Park, J., Levic, D.S., Sumigray, K.D., Bagwell, J., Eroglu, O., Block, C.L., Eroglu, C., Barry, R., Lickwar, C.R., Rawls, J.F., Watts, S.A., Lechler, T., Bagnat, M., Lysosome-Rich Enterocytes Mediate Protein Absorption in the Vertebrate Gut, 7-20.e6, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell