ZFIN Image: Zhang et al., 2019, Figure 4

Image

Figure Caption

Figure 4

A zebrafish model of Farber disease. (a) Schematic of asah1a/b^−/− zebrafish generation and propagation. (b) DNA fragment analysis demonstrating presence of altered alleles. The 30-bp difference in the asah1b spectra (rather than the noted 20-bp deletion) is due to presence of different polymorphisms across the introns of the WT and KO alleles (Fig. S2b, see Fig. S3 for sequencing of full cDNA). (c) asah1a/b^−/− (DKO) and asah1b^−/− (SKO) zebrafish at 3.5 months. (d) Length of DKO and SKO zebrafish at 3.5–4 months. Zebrafish length measurements: Student t-test, SEM, combined data from n = 13 SKO and n = 12 DKO fish, fish within each data set (n = 4–5 SKO, n = 4 DKO) are age-matched. ***p < 0.001.

Figure Data

Phenotype details

Acknowledgments

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