ZFIN Image: Castro-Sánchez et al., 2019, Figure 6

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Figure Caption

Figure 6

Representative images of KVs and comparison of average cilia length corresponding to BBS5 conditions. Projections of confocal z-stacks of Alexa fluor 594 and Dapi stained cilia located in Kupffer’s vesicle (KV) from control (A) and bbs5 MO-injected embryos (B), bbs5 MO-injected embryos plus WT capped-mRNA (C) and bbs5 MO-injected embryos plus BBS5 new changes (R138C, F180fs, N184S) capped-mRNA. (D–F) Note the reduction of the cilia in bbs5 MO and BBS5 variants R138C and N184S capped-mRNA co-injected embryos. (G) Comparison of average cilia length. *p < 0.01, **p < 0.001. Scales bars: 10 µm.

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Acknowledgments

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