ZFIN Image: Tuttle et al., 2019, Figure 2

Image

Figure Caption

Figure 2 Ret loss-of-function alters pioneer axon growth cone morphology and protrusion.

(A,B) Lateral view, live image of collective pioneer axon terminals of wild-type siblings (A) and ret^hu2846 mutants (B) labeled by neurod:EGFP transgene at 30 hpf. (A’,B’) Individual pioneer axons labeled by mosaic expression of neurod5kb:mCherry plasmid (Arrowheads = examples of counted filopodia). (C,D) Quantification of distal collective axon terminal volume (C) or individual pioneer growth cone volume (D). ret^hu2846 mutants have significantly reduced collective axonal and individual growth cone volume. (E,F) Quantification of number (E) and length (F, n = filopodia counted) of filopodia (≥1 μm) per individual pioneer axon. Note that ret^hu2846 mutants have significantly reduced number of filopodia but not length. Error bars represent S.E.M.,**=p < 0.01, ***=p < 0.001, N.S. = not significant.

10.7554/eLife.46092.007Figure 2—source data 1.Individual embryo scoring data for scoring of growth cone and filopodia metrics in <italic>ret</italic> mutants.

These data can be opened with Microsoft Excel or with open-source alternatives such as OpenOffice.

Figure Data

Phenotype details

Acknowledgments

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