ZFIN Image: Cayuso et al., 2019, Figure 4.

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Figure Caption

Figure 4. Increased tension selectively restores boundary expression of <italic>rfng</italic> in <italic>epha4</italic> and <italic>ephrinb3</italic> mutants.

(A–K) Knockdown of mypt1 increases rfng expression at hindbrain boundaries in control embryos (F) and restores rfng expression at specific boundaries in ephrinb3^-/- (G), epha4^-/- (H), epha4^Δ651 (I) and epha4^KD (J) mutants compared to uninjected controls (A–E). (K) Percentage of embryos showing rfng expression at the different boundaries for: control (n = 38); MO-mypt1 (45); epha4a^-/- (13); epha4a^-/-MO-mypt1 (42); efnb3b^-/- (14); efnb3b^-/-MO-mypt1 (17); epha4a^Δ651 (15); epha4a^Δ651 MO-mypt1 (22); epha4a^KD (13); epha4a^KD MO-mypt1 (27). Dorsal views, anterior to the top. Arrowheads indicate normal boundary expression of rfng, while asterisks indicate reduction or absence of rfng expression at boundaries. Scale bar: 50 μm.

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Acknowledgments

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