ZFIN Image: Wang et al., 2019, Fig. 6.

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Figure Caption

Fig. 6.

Heat shock-induced cell death coincides with decreased level of p-eIF2α and reduced number of stress granules in fish larvae. (A) Representative western blots showing the expression of phosphorylated eIF2α (p-eIF2α), total eIF2α (t-eIF2α) and actin after 10 min heat shock in the brain of 1, 3 and 11 dpf GFP–G3BP1 knock-in zebrafish. (B–D) Quantification of the expression of t-eIF2α relative to actin (B), p-eIF2α relative to actin (C) and p-eIF2α relative to p-eIF2α (D). Values represent mean±s.e.m. Data from three independent experiments, with n=15–20 zebrafish brains pooled for protein analysis for each condition. *P≤0.05, **P≤0.01 by unpaired Student's t-test. (E) Representative images showing stress granule formation and cell death (revealed by TUNEL staining, red) in the epidermis of 1, 3 and 11 dpf GFP–G3BP1 knock-in zebrafish exposed to 42°C for 20 min. n=4–5 zebrafish at each age with similar results. Scale bar: 10 µm.

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Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.