Injected tracers are taken up by parenchymal cells.
(A) Maximum intensity projections (3 µm thick) of the midbrain of membrane labeled (turquoise; Tg(actb2:MA-Citrine) transgenic 3 dpf fish after 10 kDa Dextran injection (green) reveals several parenchymal cells outside of the vasculature (magenta) that contain Dextran within the cell membrane (outlined in white). (B) Maximum intensity projections (5 µm thick) of the midbrain of nuclear labeled (magenta; Tg(actb2:Has.H2B-Scarlet) transgenic fish after 10 kDa Dextran injection (green) reveals several parenchymal cells that are double labeled with Dextran and nuclear signal (outlined in white), further indicating that injected tracers are taken up by select parenchymal cells. Scale bars represent 50 µm.
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