ZFIN Image: Young et al., 2019, Figure 4.

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Figure Caption

Figure 4. Exon five coding <italic>tcf7l2</italic> variant represses Wnt activity induced by <italic>FLAG-Ax2</italic> or <italic>VP16-TCF7L2</italic> in luciferase assays.

Bar plots showing luciferase reporter assay results expressed in relative light units. HEK293 cells were transiently co-transfected with luciferase reporter constructs indicated beneath the X-axis, (A–F) FLAG-Ax2 (except for first bars), (G–L) VP16-TCF7L2 DNA (except for first bars) and 4L-tcf7l2 DNA (+4L; 3^rd bars), or 45L-tcf7l2 DNA (+45L; 4^th bars) or tcf7l2-AA DNA (+AA; 5^th bars). Control experiments show only background luciferase activity with no transfected plasmids (1^st bars). Figures in the bars indicate the percentage size of that bar relative to transfection with either FLAG-Ax2 or VP16-TCF7L2 alone (2^nd bar). Error bars are mean ± SD n = 3 (experiments were performed twice), P values from unpaired t tests comparing FLAG-Ax2 or VP16- TCF7L2 control condition with tcf7l2 variant co-transfections. Comparisons with no statistical significance are not marked.

Acknowledgments

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