ZFIN Image: Morishita et al., 2019, Figure 1

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Figure Caption

Figure 1 Loss of vmp1 in zebrafish causes lethality around 9 days post fertilization and defective autophagy. (A) Schematic representation of the Cas9-gRNA-targeted site in the zebrafish vmp1 genomic locus. The protospacer-adjacent motif (PAM) sequence is shown in red. The targeted site is underlined. A 7 bp deletion in the mutated allele is shown. (B) External appearance of 6-dpf vmp1^+/+, vmp1^+/-, and vmp1^-/- zebrafish. Magnified images of the indicated regions are shown in the right panels. Dashed lines indicate abnormal deposits in the liver and intestine. Data are representative of four independent experiments. (C) Survival rate (% of total fish) of vmp1^+/+ (n = 7), vmp1^+/- (n = 30), and vmp1^-/- (n = 11) zebrafish. Data are representative of two independent experiments. (D) Representative images of GFP-LC3 signals in the midbrain, spinal cord, and skeletal muscle of 3-dpf vmp1^+/- and vmp1^-/- zebrafish injected with GFP-LC3 mRNA. Data are representative of two independent experiments. Scale bars, 10 μm and 1 μm in the inset. (E) Immunoblotting of LC3 and β-actin in two 7-dpf vmp1^+/- and vmp1^-/- zebrafish. Data are representative of two independent experiments.

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Acknowledgments

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