Fig. 4

Fig. 4

a Diagrams of zRbm14b and its mutants. Detailed mutation sites in zRbm14b18S are provided in Supplementary Fig. 3a. b Subcellular localizations of GFP-tagged zRbm14b, zRbm14b18S, and zRbm14bIDR in HeLa cells. HeLa cells were transfected for 48 h with the intact plasmids for the in vitro mRNA productions (c). Nuclear DNA was stained with DAPI. Arrows indicate typical nuclear puncta. c, d zRbm14b18S and zRbm14bIDR failed to rescue the dorsalized phenotypes of rbm14 morphants. Zebrafish embryos at the one-cell stage were co-injected with 14-MOs (total 8 ng per embryo) and in vitro-transcribed mRNA (300 pg mRNA per embryo) coding for GFP, GFP-zRbm14b, GFP-zRbm14b18S, or GFP-zRbm14bIDR (also see Supplementary Figs. 1f and 4a). Embryos injected with 8 ng of ctrl-MO served as a negative control. The samples were imaged at 72 hpf (c). The quantification results (d), based on the criteria and examples in Fig. 1e and represented as mean ± SD, were from three independent experiments. Student’s t-test against the GFP mRNA-injected populations: n.s., no significance; **P < 0.01; ***P < 0.001. Total number of embryos analyzed are listed over each histogram