Figure 3

Figure 3

(a) Effect of Rpl10a deficiency on embryonic development. Lateral views of rpl10a MO-injected embryos and rescue phenotype observed at 25 and 50 hpf. At 25 and 50 hpf, smaller eyes, yolk sac extensions, bent tails, and developmental delays were observed in rpl10a knockdown. At 50 hpf, deficient embryos displayed smaller eyes with reduced pigmentation and edema (arrowheads). The MO^sp + mRNA injected embryos displayed rescue phenotypes. (b) The phenotype of rpl10a MO^aug (n = 90), MO^sp (n = 105), and MO^sp + mRNA (n = 54) injected embryos was evaluated compared to wild-type (n = 107) at 25 hpf. The morphologic abnormalities include a thinner yolk sac extension, a shorter body length, and a bent tail, whereas the normal wild-type embryos showed a normal yolk sac extension and a straight tail. The result was presented in the percentage of normal (black bar) and abnormal (white bar) embryos in each group.