FIGURE 3

FIGURE 3

Behavior of reln^+/– and reln^–/– zebrafish. (A–C) Both genotypes shoal normally. (A) Inter-individual distance (Kruskal–Wallis test with Dunn’s multiple comparisons test: chi-square = 3.71, p > 0.99 for reln^+/– and p = 0.36 for reln^–/–); (B) Polarization (Kruskal–Wallis test with Dunn’s multiple comparisons (test: chi-square = 0, p > 0.99 for both genotypes) and (C) velocity [one-way ANOVA Dunnett’s multiple comparisons test: F(2,3) = 5.498, p = 0.09 for WT, p = 0.31 for reln^+/– and p = 0.07 for reln^–/–]. n = 2 groups of 5 WT, n = 2 groups of 5 reln^+/– and n = 2 groups of 5 reln^–/–. (D–F) Both reln^+/– and reln^–/– behave similarly to WT in the open field test. (D) Time at the side of the tank [thigmotaxis, one-way ANOVA Dunnett’s multiple comparisons test: F(2,33) = 1,687, p = 0.20, p = 0.13 for WT vs. reln^+/– and p = 0.50 for WT vs. reln^–/–]; (E) time spent in the center of the tank [one-way ANOVA Dunnett’s multiple comparisons test: F(2,33) = 1,431, p = 0.25 for WT, p = 0.25 for reln^+/– and p = 0.99 for reln^–/–]. (F) Locomotion is not affected in the open field test [one-way ANOVA Dunnett’s multiple comparisons test: F(2,33) = 1,625, p = 0.21, p = 0.14 for reln^+/– and p = 0.61 for reln^–/–]. n = 12 WT, n = 12 reln^+/–, n = 12 reln^–/–. (G,H) Both genotypes exhibit normal anxiety-like behavior. (G) Time at the bottom of a novel tank (Kruskal–Wallis test with Dunn’s multiple comparisons test: chi-square = 0,5705, p = 0.93 for reln^+/– and p > 0.99 for reln^–/–); (H) locomotion in a novel tank [one-way ANOVA Dunnett’s multiple comparisons test: F(2,35) = 1,54, p = 0.23 for WT, p = 0.75 for reln^+/– and p = 0.43 for reln^–/–) n = 13 wild-type, n = 13 reln^+/–, n = 12 reln^–/–. (I) No difference in aggression levels between WT, reln^+/– and reln^–/– (Kruskal–Wallis test with Dunn’s multiple comparisons test: chi-square = 3,372, p = 0.97 for reln^+/– and p = 0.13 for reln^–/–) n = 12 wild-type, n = 12 reln^+/–, n = 8 reln^–/–. Mean ± SEM.)