ZFIN Image: Liu et al., 2019, Fig. S4

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Figure Caption

Fig. S4

(A) Time-lapse confocal images showing DFC migration in wild-type and cxcr4a^um20mutant embryos on a Tg(sox17: GFP) background from 75%–90% epiboly stages. Scale bar, 50 μm. (B) Sox17 expression was examined by in situ hybridization in wild-type and cxcr4a^um20 mutants at the 75% epiboly stage. (C–D) Wild-type and cxcr4a-deficient Tg(sox17: GFP) embryos were harvested at the 10-somite stage for immunostaining. KV cells were labeled using an antibody against GFP. Expression of the basal–lateral marker E-cadherin (C) and the apical marker aPKC (D) were visualized using the indicated antibodies. Scale bar, 20 μm. aPKC, atypical protein kinase; DFC, dorsal forerunner cell; GFP, green fluorescent protein; KV, Kupffer’s vesicle; sox, SRY-box transcription factor; Tg, transgene.

Acknowledgments

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