ZFIN Image: Mathewson et al., 2019, Fig. 2

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Figure Caption

Fig. 2

Vangl2 and Fzd3a localization in the floorplate. (A–B) Live lateral views of 48hpf (A) Tg(shh:gal4); Tg(uas:GFP-Vangl2) and (B) Tg(shh:gal4); Tg(uas:Fzd3a-GFP) single expressing floorplate cells. Asterisks indicate positions of fusion protein (green) concentration along the apical membrane. (C) Schematic: diagram indicating how total fusion protein localization was measured on anterior vs. posterior cell halves. Graph: quantitation of anterior vs. posterior fusion protein localization in isolated expressing cells. Each dot represents the ratio of the mean fluorescence level within a single cell anterior half divided by the mean fluorescence level of its posterior half. N = 57 embryos, 152 cells; **p = 0.0035; *p = 0.0266; significance was determined with a Kruskal-Wallis test with Dunn's multiple comparison. (D) Schematic: diagram indicating how fusion protein localization was measured on anterior vs. posterior membranes. Graph: quantitation of anterior vs. posterior fusion protein membrane localization in isolated expressing cells. Each dot represents the ratio of the mean fluorescence level along the anterior membrane a single cell divided by the mean fluorescence level along its posterior membrane. N = 39 embryos, 106 cells; *p = 0.0154; significance was determined with a Kruskal-Wallis test with Dunn's multiple comparison. (E) Live lateral views of a 48hpf Tg(shh:gal4); Tg(uas:Fzd3a-GFP); Tg(uas:mAppleCAAX) dual transgene expressing floorplate cell. Inset: magnified view of the posterior apical cellular membrane where Fzd3a-GFP (green, asterisk) is concentrated near the base of the primary cilia (red apical extension, arrow). Approximate cell boundaries, as determined by mAppleCAAX expression, indicated by white dotted lines. (F) Schematic: diagram indicating how apically localized cytosolic Fzd3a-GFP levels were quantified (see methods) Graph: correspondence of Fzd3a-GFP peak localization with primary cilium position. Each point corresponds to measurements from a single cell. r² = 0.79. Anterior to left in all images. Scale bars: 5 μm.

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 452(1), Mathewson, A.W., Berman, D., Moens, C.B., Microtubules are required for the maintenance of planar cell polarity in monociliated floorplate cells, 21-33, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.