Fig. S4

Fig. S4

Tg(her4.2:dRFP) embryos injected with 9-HSA display increased RFP expression from 2 to 3 days post-fertilization, related to Figure 3. Tg(her4.2:dRFP) embryos were injected at 1-cell stage with either 9-HSA or DMSO. The effect of the injection on the expression level of her4.2 was assessed at 1, 2 and 3 days post-fertilization (dpf). At 1 dpf, no difference could be observed between the retinae of 9-HSA and DMSO injected embryos (A – A’). At 2 and 3 dpf, an increased expression of her4.2:dRFP could be observed in the retinae of 9- HSA in comparison to DMSO injected embryos of the same developmental stage (B – C’). (D) Quantification of the her4:dRFP fluorescent intensity at 3 dpf showed a significant increase in 9-HSA injected retinae of the dRFP signal compared to the control DMSO injected retinae (35.71 SEM ± 1.038 AU for 9-HSA and 24.79 SEM ± 2.005 AU for DMSO injected embryos, p-value: 0.0168, unpaired Welch’s t-test). Scale bar (A – C’) = 20 μm.