ZFIN Image: Whitesell et al., 2019, Fig. 9

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Figure Caption

Fig. 9

foxc1 acts upstream of acta2 in vSMC differentiation. A-D) Ablation of foxc1b and acta2 positive cells using Metronidazole (MTZ) from 3 to 4 dpf. Embryos are heterozygous for foxc1b- or acta2:Gal4 and UAS:NTR-mCherry. Ventral views show smooth muscle coverage along the ventral aorta in controls (A and C), but largely absent in MTZ treated embryos (B, D). E-F) Ventral views of mural cell marker mRNA (acta2 and pdgfrβ) in foxc1b:Gal4; UAS:NTR-mCherry embryos show a reduction in acta2 and increase in pdgfrβ in MTZ treated embryos (F) versus controls (E). G-H) Expression of acta2 is reduced in MTZ treated acta2:Gal4; UAS:NTR-mCherryembryos versus controls, while pdgfrβ expression is unchanged. I-L) Expression of foxc1b mRNA is reduced in MTZ treated foxc1b:Gal4; UAS:NTR-mCherry embryos compared to controls, while foxc1bmRNA is unchanged in controls and MTZ treated acta2:Gal4; UAS:NTR-mCherry embryos. Green hearts in A-H are due to the transgenesis marker (myl7:EGFP). Scale bar represents 50 μm.

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Acknowledgments

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Reprinted from Developmental Biology, 453(1), Whitesell, T.R., Chrystal, P.W., Ryu, J.R., Munsie, N., Grosse, A., French, C.R., Workentine, M.L., Li, R., Zhu, L.J., Waskiewicz, A., Lehmann, O.J., Lawson, N.D., Childs, S.J., foxc1 is required for embryonic head vascular smooth muscle differentiation in zebrafish, 34-47, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.