ZFIN Image: Sharma et al., 2019, Fig. 3

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Figure Caption

Fig. 3

col1a2⁺ cells contribute to muscle growth. (A) Schematics of Cre-mediated lineage tracing experiments. (B) col1a2^Cre-ERT2; ubi:Switch embryos were pulsed with 4-OHT for 2 h at 3 dpf to induce EGFP excision, and imaged for 4 days. Color-coded depth projections of mCherry expression are shown. ‘Switched’ mCherry⁺ MPCs (arrowheads) at the +24 h time point generated new mCherry⁺ myofibers (arrows) starting from the +48 h time point. n=20 embryos. (C) ‘Switched’ col1a2^Cre-ERT2; ubi:Switch fish were sectioned and imaged at 4 wpf (n=4 fish at 12 mm SL) and 1 ypf (n=4 fish at 30 mm SL). Transverse sections (stitched confocal tile scans) with expanded views of boxed regions are shown. Arrows denote mCherry⁺ myofibers and arrowheads indicate the mCherry⁺ skin. Scale bars: 50 µm (B,C) and 200 µm (full views in C).

Acknowledgments

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