ZFIN Image: Li et al., 2019, Fig. 1

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Figure Caption

Fig. 1

Structure of zebrafish ar gene and DNA construct for liver-specific knockout. (A) Schematic representation of eight arexons to cover the complete coding region. The sequence (underlined) and position of the ar gRNA target at the first exon are indicated. PAM sequence (Cas9 binding site) is in Red. (B) Schematic illustration of the liver-specific CRISPR construct. Tol2, Tol2 transposon; P_cmlc2: cmlc2 promoter; ar gRNA, ar guide RNA; pA: poly A signal; P_fabp10: fabp10a promoter. (C) The negative (left) and positive (right) F1 larvae from an L-ARKO founder transgenic zebrafish. The heart is marked with GFP expression as a screening marker.

Acknowledgments

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