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Fig. S5
Comparison between in situ RNA Localization and Brain Activity, Related to Figure 4
Stacks of all RNA-FISH images are available on http://stackjoint.com/basic/ and are tagged as “Thyme, 2018.” Probe sequences are available on http://genepile.com/scz_gwas108. At least three brains were imaged for each probe to confirm that the presented pattern was consistent (data available upon request). Brain areadescriptions are based on the Z-Brain masks (stackjoint.com/zbrain). znf536: forebrain showed in situ and phosphorylated-Erk (pErk) brain activity signal. grin2aa: cerebellum showed in situ and activity signal. elfn1a: retinal arborization field AF7, tectum, and forebrain showed in situ and activity signal. foxg1: forebrain showed in situ and activity signal. gria1a: forebrain showed in situ and activity signal. cacna1c:cerebellum and forebrain showed in situ and activity signal. hcn1:hindbrain showed in situ and activity signal. egr1: forebrain showed in situ and activity signal. roraa: tectum and hindbrain showed in situ and activity signal. nrgn: forebrain and hypothalamic cells showed in situ and activity signal. snap91: activity signal correlated to in situ signal showing expression throughout the brain. shisa9b: forebrain showed in situ and activity signal. znf804a: activity signal correlates to in situ signal showing expression throughout the brain. lrrn3: activity signal correlated to in situ signal showing expression throughout the brain. csmd1: activity signal correlated to in situ signal showing expression throughout the brain. cnnm2b: strong in situ signal in forebrain and torus semicircularis did not correlate to activity signal in tectum, retinal arborization field AF7, and hindbrain. csmd3: strong in situ signal in retinal arborization field AF7, tectum, and hindbrain did not correlate to diffuse and minimal activity signal. tcf4: strong in situ signal in forebrain and midbrain did not correlate to strong activity signal in tectum. luzp2:Strong in situ signal in tectum and cerebellum did not correlate to activity signal in hindbrain. tle3a: strong in situ signal in forebrain and tectum did not correlate to strong activity signal primarily in hindbrain. clcn3: strong in situ signal throughout the brain did not correlate to activity signal mainly in forebrain. grin2ab: strong in situsignal throughout the brain did not correlate to activity signal primarily in the tectum, retinal arborization field af7, and hindbrain. bcl11b:strong in situ signal throughout the brain did not correlate to activity signal in forebrain and cerebellum.
Reprinted from Cell, 177(2), Thyme, S.B., Pieper, L.M., Li, E.H., Pandey, S., Wang, Y., Morris, N.S., Sha, C., Choi, J.W., Herrera, K.J., Soucy, E.R., Zimmerman, S., Randlett, O., Greenwood, J., McCarroll, S.A., Schier, A.F., Phenotypic Landscape of Schizophrenia-Associated Genes Defines Candidates and Their Shared Functions, 478-491.e20, Copyright (2019) with permission from Elsevier. Full text @ Cell