ZFIN Image: Fontenas et al., 2019, Fig. 1

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Figure Caption

Fig. 1

Small-Molecule Screen Identifies Compounds that Disrupt OPC Migration at the MEP TZ

(A) Schematic of a transverse view of the MEP TZ. MEP glia (MEPg; green) prevent OPC (yellow) exit from the spinal cord (gray). OPC processes (arrow) contact MEP glia but are repelled. Without MEP glia, OPCs migrate onto peripheral nerves.

(B) Low-magnification images showing lateral views of negative (top) and positive (bottom) controls at 3 dpf in olig2:dsred larvae. Peripheral olig2⁺ cells are denoted with yellow arrowheads.

(C) Images of control and validated hits in 3 dpf olig2:dsred larvae showing ectopic OPCs (arrowhead) along spinal motor nerves.

(D) Mean ± SEM of peripheral OPC count for larvae injected with injection buffer (control) or TeNT mRNA at 3 dpf. p = 0.0013; n = 14 control, n = 23 TeNT.

Scale bars, 100 μM in (B) and 20 μM in (C).

Figure Data

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Acknowledgments

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