IMAGE

Fig. 5

ID

ZDB-IMAGE-190820-10

Genes

Antibodies

Ab2-adora2a

Source

Figures for Fontenas et al., 2019

Image

Figure Caption

Fig. 5

A2a AR Inhibition Reduces Neural Activity of Spinal Cord Neurons

(A) Lateral views of a 60 hpf WT olig2:dsred;sox10:eos larva stained with an A2a AR antibody. Top: spinal cord (CNS) with A2a expression in a subset of olig2⁺ motor neurons (asterisks). Bottom (PNS): peripheral spinal nerves with A2a expression in motor (closed arrowheads) and sensory (open arrowheads) axons.

(B) adora2ab mRNA expression at 72 hpf in the spinal cord of a WT larva. Yellow asterisks denote motor neurons.

(C) Twenty-five hours post-fertilization olig2:egfp embryo labeled with SCH-red fluorescent antagonist. Arrowheads mark spinal cord olig2⁺/SCH-red⁺ motor neurons.

(D) Mean ± SEM of the fold change in ΔF/F₀ over time per individual firing event for larvae treated with DMSO (n = 8 neurons from eight larvae) and SCH-58261 (n = 11 neurons from ten larvae); p = 0.01. Scale bars, 20 μM.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.