Fig. 4

(A) Single-plane brightfield images of wild-type, heterozygous and stat3 mutant larvae showing tissue regrowth of caudal fins at 72 hr post tail transection (hptt) and (B) corresponding quantification of area of tissue regrowth. Values are arithmetic means and SE from three biological replicates. p values obtained by analyzing ranks due to residuals not being normally distributed. N = 41–50 larvae from het incross, per biological repeat, followed by genotyping. ****p<0.0001; black and gray * depict p values in comparison to wild-type and heterozygous, respectively. (C) Z-projections of multiphoton microscopy images showing the location of vimGFP-positive cells and SHG fiber organization in wild-type and stat3mutant larval caudal fins at 72 hptt. Asterisk denotes region just posterior to tip of notochord. Scale bar is 50 microns. (D) Quantification of the number of vimGFP-positive cells at the wound edge in wild-type and stat3 mutant larvae. Values are least square means and SE from three replicates; Total N = 28–32 larvae per treatment. ***p<0.001 (E) Graph comparing the contour length of the wound edge, measured using SHG images, between wild-type and stat3 mutant larvae. Values are arithmetic mean and SE from three experimental replicates with associated p values obtained by analyzing ranks due to residuals not being normally distributed. ****p<0.0001. Total N = 28–32. (F) Sum-projections of z-stacks acquired by spinning disk confocal microscope using Tg(vim:GFP) larvae, following tail transection at 72 hptt (6 dpf) in E3 medium only (untreated, UT) or in the presence of 25 µM ALK5 (TGFβRI) inhibitor or 0.5% DMSO as vehicle control. (G) Corresponding quantification of area of tissue regrowth. Values are arithmetic means and SE from three biological replicates. p values obtained by analyzing ranks due to residuals not being normally distributed. (H) corresponding quantification of vimGFP-positive cells at the wound edge. Values are least square means and SE from three biological replicates, with associated p values. Total N = 36–43 larvae for each treatment in (G) and (H). ****p<0.0001; black and gray * depict p values in comparison to untreated and DMSO-treated respectively.