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Fig. 5
Lateral protrusive activity, radial protrusive activity, and mitotic events in lcp1:EGFP-CAAX EVL cells.
A. Time-lapse imaging of EVL lateral protrusive activity (Movie S2). The view is of the animal cap, and several membrane projections are false-colored green. A bright EVL cell extends a lamellipodial projection (white arrow) under the nucleus of an adjacent dark cell. The projection then breaks up into smaller filopods and vesicles.
B. Time-lapse imaging of EVL radial protrusive activity (Movie S3). The confocal volume has been segmented to remove the deep cell layer, inverted to show EVL membranes extending upwards, and recolored using an automated depth-coding scheme. Blue voxels = apical extreme; red voxels = basal extreme. Radial protrusions appear as yellow-to-red spikes (white arrows) that extend and retreat every few minutes.
C. Time-lapse imaging of EVL mitosis. The dividing cell is false-colored green. EGFP expression levels vary from cell to cell but are stable throughout the cell cycle. When mitoses occur, daughters inherit the parental signal. Timestamp for all panels = minutes:seconds.
Reprinted from Gene expression patterns : GEP, 32, Baumgartner, E.A., Compton, Z.J., Evans, S., Topczewski, J., LeClair, E.E., Identification of regulatory elements recapitulating early expression of L-plastin in the zebrafish enveloping layer and embryonic periderm, 53-66, Copyright (2019) with permission from Elsevier. Full text @ Gene Expr. Patterns