ZFIN Image: Baumgartner et al., 2019, Fig. 3

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Figure Caption

Fig. 3

Transgenic expression in lcp1:EGFP-CAAX embryos.

A. 30% epiboly, transgenic embryo in equatorial view. The EVL shows weak, variegated EGFP expression.

B. 70% epiboly, transgenic embryo in equatorial view. Signal intensifies as the EVL spreads over the yolk.

C. 70% epiboly, cutaway view of a single focal plane. Only EVL cells are EGFP+, in contrast to the deep cells (DC) and yolk syncytial layer (YSL).

D. Live embryo at the 15-somite stage. lcp1:EGFP-CAAX labels the periderm, the outermost epithelial layer.

E. Magnification of the tailbud in D, showing variegated EGFP in adjacent peridermal cells.

F. Fixed embryo at 1 dpf, with phalloidin-568 counterstain. A patch of label-retaining peridermal cells (green) lies over the developing trunk musculature (red).

Acknowledgments

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Reprinted from Gene expression patterns : GEP, 32, Baumgartner, E.A., Compton, Z.J., Evans, S., Topczewski, J., LeClair, E.E., Identification of regulatory elements recapitulating early expression of L-plastin in the zebrafish enveloping layer and embryonic periderm, 53-66, Copyright (2019) with permission from Elsevier. Full text @ Gene Expr. Patterns