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Fig. S3
In vivo imaging of individual tectal neurons shows that both primary and secondary cell death occur after brain injury. (A,C,E) Confocal images of theoptic tectum of H2A:GFP transgenic animals, where tectal neurons labelled through injection of elavl3:memTdTomato plasmid DNA die through primary cell death (A), survive (C), or die through secondary cell death (E) after mechanical injury. Scale bars, 50 μm. (B,D,F) Close-up of neurons indicated in (A), (C) and (E). White arrow indicates pyknotic nucleus. Scale bars, 10 μm.
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