ZFIN Image: Villani et al., 2019, Fig. S3

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Figure Caption

Fig. S3

– Gastrosome enlargement in siRNA mediated Slc37a2 knock-downs in mammalian macrophages-

Related to Figure 7. (A) RAW264.7 macrophages unfed (upper panels) or fed with HK H2B mCherry cells and fixed 24hrs after apoptosis induction (lower panels). Untreated controls (left column), cells transfected with a positive control for transfection (scr-siRNA FITC conjugated (green) middle column) and cell transfected with an siRNA targeting Slc37a2 (right column). The yellow arrowhead indicates the gastrosome in fed cells. Scale bars 5μm. (B) V-plot showing velocity of trunk macrophages in wild type (N=6; n=146) and blbNY007 (N= 6; n=180). P value = 0.0115 (Wilcoxon rank-sum test). (C, movie 10) Representative images of the response of trunk macrophages to injury. Upper panel macrophages in wild type and lower panel macrophages in blbNY007. 5 minutes after injury induction (left) and 2 hours after injury induction (right). Scale bar 30 μm.

Acknowledgments

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Reprinted from Developmental Cell, 49(1), Villani, A., Benjaminsen, J., Moritz, C., Henke, K., Hartmann, J., Norlin, N., Richter, K., Schieber, N.L., Franke, T., Schwab, Y., Peri, F., Clearance by Microglia Depends on Packaging of Phagosomes into a Unique Cellular Compartment, 77-88.e7, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell