Fig. 1

Fig. 1

Characterization of bubblebrain, a Mutant with Bloated Microglia

(A and B) Neutral red staining in a 4 dpf wild-type (A) and blb^NY007 (B) embryos.

(C and D) Microglia labeled with Tg(Apo-E:lynGFP) in a 4 dpf wild-type (C) and blb^NY007 (D) embryo.

(E and F) Microglia cell labeled with Tg(spi1b:Gal4,UAS:GFP) in a 4 dpf wild-type (E) and blb^NY007 (F) embryo. Scale bar, 10 μm. 

(G) Quantification of the “bubble” size in blb^NY007 embryos from 2.5 to 4.5 dpf in optic tectum (OT, red plots) and in the hindbrain (HB, orange plots). Whiskers: 5th/95th percentile, p value < 0.0001.

(H) Quantification of apoptotic neurons visualized by Acridine Orange in wild-type (n = 20), blb^NY007 (n = 17) and in wild-type (n = 18) and blb^NY007 (n= 19) injected with pU1 morpholino to deplete microglia in 3.5 dpf embryos; representative images in (J) and (K). Whiskers: 5th/95th percentile, p value < 0.0001.

(I) Quantifications of phagocytic events per cell per hour in wild-type (n = 50; n = 47) and blb^NY007 (n = 61; n = 77) embryos at 3.5 dpf and 4 dpf, respectively. Whiskers: 5th/95th percentile, p value < 0.0001.

(J and K) Dorsal view of a 3.5 dpf wild-type (J) and blb^NY007 (K) embryo injected with pU1 morpholino to deplete microglia and stained with Acridine orange to visualize apoptotic neurons; quantifications in (H).

(L) Microglia cell labeled with Tg(mpeg:GFP-caax) and Tg(NBT:secA5-BFP) to visualize apoptotic neurons in a 4 dpf blb^NY007 embryo.