ZFIN Image: Tobia et al., 2019, Figure 4

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Figure Caption

Figure 4

ackr3b knockdown affects ISV development and endothelial filopodia protrusions. Tg(kdrl:EGFP) embryos injected with std-MO (left panels) or ackr3b-MO1 (right panels) were photographed at 26 hpf (B,C) and 48 hpf (D,E) under an epifluorescence microscope. (B–E) High magnifications of the trunk region highlighted by the dotted box in (A) Asterisks in (C,E) indicate aberrant sprouts in ackr3b-MO1-injected embryos. Vasculogenesis occurs normally in ackr3b morphants as indicated by the presence of DA and PCV (brackets in B,C). (F–I) Confocal microscopy analysis of 28 hpf Tg(kdrl:EGFP) zebrafish embryos injected with std-MO (F,G) or ackr3b-MO1 (H,I). Note the aberrant filopodia protrusions from stalk cells in ackr3b morphants (arrows in I) when compared to control embryos. (G,I) High magnification of the region highlighted by dotted boxes in (F,H), respectively. (A–E) Scale bar: 100 μm. (F–H) Scale bar: 25 μm.

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Acknowledgments

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