ZFIN Image: Guarino et al., 2019, Figure 1

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Figure Caption

Figure 1

Immunoreactivity of human YB-1 antibodies against zebrafish YB-1. (a) confocal immunofluorescence analysis of PAC2 cells stained with human YB-1 antibodies (α-YB-1 C-ter and α-YB-1 N-ter), nuclei were stained with DAPI (blue); (b) western blot of PAC2 total (TOT), Cytoplasmic (C) and nuclear (N) protein extracts analysed with α-YB-1 N-ter and α-YB-1 C-ter antibodies; Histone H3 and ß-tubulin were used as loading controls for the nuclear and cytoplasmic extracts, respectively. Each panel is assembled from cropped western blotting images (see Supplementary Material file for the original images).

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