Figure 5

Figure 5

Islet K_ATP‐GOF results in profound diabetes. (A) Transgenic strategy for conditional K_ATP‐GOF expression in zebrafish islet. mCherry, expressed under insulin promoter control (upper panel), is excised and K_ATP‐GOF construct is expressed, after (lower left panel) Cre recombination. A F2 larva is shown in the right lower panel, with the islet highlighted in the yellow circle. (B) GFP (left column) and bright‐field (right column) images of dissected islets from adult control, uninduced K_ATP‐GOF and induced K_ATP‐GOF fish (images taken at 12 × ). (C) Random blood glucose levels in controls (N = 25), uninduced (N = 15), and induced (5 days heat‐shock, N = 24) K_ATP‐GOF zebrafish. In control and induced fish, blood glucose were measured 2 days after the last heat shock. (D) Time course of change in blood glucose following K_ATP‐GOF induction. (E) Glucose levels in adult (10 week old) K_ATP‐GOF fish are similarly elevated, whether induced as larvae (N = 4), or as adults (N = 15).