ZFIN Image: Balciuniene et al., 2013, Figure 1

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Figure Caption

Figure 1

Design of GBT-B1 trap. (A) Parental vector GBT-R15 [27,28]. cSA denotes carp β-actin splice acceptor, ^mRFP denotes AUG-less mRFP, and zp(A) denotes zebrafish β-actin 3’ untranslated region and transcriptional termination / polyadenylation cassette. Tol2 5’ and Tol2 3’ are miniTol2 transposon arms as described in [37]. (B) Features of GBT-B1 trap. ^Gal4-VP16 denotes AUG-less Gal4-VP16, and the 14XUAS:eGFP cassette is from [34]. (C) Embryos injected with GBT-B1 without Tol2 transposase mRNA. (D) Embryos injected with GBT-B1 and Tol2 transposase mRNA.

Acknowledgments

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