Fig. 2

Clec14a and C1qr have a partially redundant function during angiogenic sprouting. Kdrl: GFPexpression in the trunk region imaged by fluorescent microscopy at 28 hpf (a-d), 48 hpf (e-h) and 72 hpf (i-l). a, e, i Wild-type control embryos; b, f, j clec14a−/− mutants; c, g, k Wild-type embryos injected with 10 ng of c1qr MO; d, h, l clec14a−/− mutant embryos injected with 10 ng of c1qr MO. Note the delayed intersegmental vessel sprouting in clec14a mutants (b) and c1qr MO embryos (c) at 24 hpf (arrowheads). Partial sprouts (arrowheads) and abnormal vascular connections (arrows) are apparent in the clec14a−/−; c1qr MO embryos (d, h, l). m Percentage of embryos with vascular defects. ** p < 0.01; *** p < 0.001; NS, no significance, Fisher’s exact test. Data were combined from 3 independent experiments. Error bars show standard error. n Distribution of different types of phenotypes in embryos at 48 hpf. Data were combined from two independent experiments