ZFIN Image: Pociute et al., 2019, Fig. 1

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Figure Caption

Fig. 1

Generation of clec14a mutants by TALEN-mediated genome editing. a Wild-type and clec14a^ci15 mutant sequence showing the binding regions for TALE nucleases and the 10 bp deletion in the clec14a mutants which includes the deletion of HhaI restriction site used for genotyping. b A diagram of Clec14a protein sequence which includes C-type lectin and transmembrane (TM) domains. clec14a^ci15 mutation is predicted to cause a frameshift early in the protein coding sequence starting at the amino acid 44 and would lead to a premature stop codon. c, d DNA sequencing chromatogram shows a 10 bp deletion (boxed-in wild-type sequence) in the cDNA of clec14a mutants. d, e In situ hybridization analysis for clec14a expression in wt embryos (d) and clec14a mutants (e) at 24 hpf. Note a significant reduction of clec14a expression in clec14a mutant embryos. The experiment has been replicated twice; the combined number of embryos analyzed and showing the phenotype is shown in the lower right corner

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.