ZFIN Image: Krall et al., 2019, Fig 4

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Figure Caption

Fig 4 Phenotypic and genotypic analysis following treatment of incrossed heterozygous <italic>bmp4</italic><sup><italic>st72/+</italic></sup> zebrafish with 0 μM, 0.25 μM and 0.5 μM PTC124.

Larvae were dechorionated at 6–8 hours post fertilization (hpf) prior to starting PTC124 treatment. Analysis was performed at 72 hpf and the results are shown as the mean of 3 independent experiments. Larvae were scored as wildtype (E), mild reduction/deformity in ventral tail fin (D+ or D) or severe reduction/deformity in ventral tail fin (C). WT = wildtype, het = heterozygote, HZ = homozygote. Error bars show standard error of the mean. We tested for trend in the probability of tail fin defects across the increasing doses of 0 μM, 0.25 μM and 0.5 μM PTC124 treated, groups of homozygous larvae [24]. The probability of tail fin defect decreased from 81.08% to 74.07% and 37.50% as the dose increased to 0.25 μM and 0.5 μM. The decreasing trend was statistically significant (p = 0.0002).

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Acknowledgments

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