ZFIN Image: Yang et al., 2019, Fig. 2

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Figure Caption

Fig. 2 Zebrafish <italic>cul4a</italic> regulates the primitive erythropoiesis.

aGata1 was analyzed by WISH in embryos at 6 somite injected with CoMO, cul4a-MO, cul4b-MO, cul4a/cul4b-MO, or cul4a-MO coinjected with zebrafish cul4a mRNA. b Relative gata1 expression was analyzed by qRT-PCR in embryos injected with CoMO, cul4a-MO, cul4b-MO, cul4a/cul4b-MO, or MO coinjected with cul4a mRNA, respectively. ccul4a-MO was injected into Tg (gata1: EGFP) transgenic embryos and decreased EGFP-positive cells were observed. Coinjection of cul4a mRNA rescued the reduction in gata1⁺ cells caused by cul4a MOs. d–f Effects of gata1 mRNA in rescuing hematopoietic defects in cul4a-morphant embryos as demonstrated by staining of o-dianisidine (d), WISH (e) and qRT-PCR (f) of hbbe3 probes. The number in the top right-hand corner indicates the phenotypic embryos/total embryos. qRT-PCR experiments were performed in triplicate. ***p < 0.001. All scale bars represent 250 μm

Acknowledgments

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