ZFIN Image: Pereira Piedade et al., 2019, Fig. 4.

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Figure Caption

Fig. 4.

Siah activity indirectly regulates pax2a expression. (A) WISH for pax2a after injection of Siah1, Siah1ΔR, Siah2l or Siah2lΔR±MG132 at 24 hpf. (B) qPCR results for pax2a expression at 24 hpf. *P<0.05 compared to uninjected. (C) WISH for pax2a gene expression upon co-injection of Siah1+Nlz2 or Nlz2^nxn. (D) qPCR results for pax2a expression at 24 hpf. *P<0.05 compared to uninjected, ^@P<0.05 compared to Nlz2, ^#P<0.05 compared to Siah1, ^$P<0.05 compared to Siah1+Nlz2. (E) Tg[rx3:GFP] (green) embryos injected with siah1, siah2l, siah1ΔR or siah2lΔR±100 pg Nlz2 mRNA stained for laminin (red) and DAPI (blue) at 72 hpf. Arrowheads indicate persistence of laminin. Scale bar: 50 µm. (F) Proportion of embryos with failure of fusion (1) or completed fusion (0). Region of analysis is outlined by dashed lines. *P<0.05 compared to uninjected, ^#P<0.05 compared to Siah1, ^$P<0.05 compared to Siah1+Nlz2. One-way ANOVA; P<0.0001. Dashed boxes indicate region of inset.

Acknowledgments

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