ZFIN Image: Pereira Piedade et al., 2019, Fig. 1.

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Figure Caption

Fig. 1.

Nlz2 regulates optic fissure closure by inhibiting pax2a gene expression. (A) WISH for pax2a in 24 hpf embryos injected with varying amounts of Nlz2 mRNA. OF-associated pax2a signal is decreased in response to increasing Nlz2 mRNA. (B) qPCR results at 24 hpf for pax2a expression±s.d. *P<0.05 compared to uninjected, ^$P<0.05 compared to Nlz2 50 pg. (C) 72 hpf Tg[rx3:GFP] (green) embryos injected with Pax2a, Nlz2 or Pax2+Nlz2 mRNA stained for laminin (red) and DAPI (blue). Arrowheads indicate persistence of laminin signal. Scale bar: 50 µm. (D) Proportion of embryos with failure of fusion (1) or completed fusion (0)±s.d. Region of analysis is outlined by dashed lines. *P<0.05 compared to uninjected, one-way ANOVA; P<0.0001. Dashed boxes outline region of inset.

Acknowledgments

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