ZFIN Image: Zhao et al., 2019, Fig. S6

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Figure Caption

Fig. S6

(A) sas10Δ2 and mpp10Δ10 mutant embryos did not show abnormal apoptotic activities. TUNEL assay was performed on cryosections of WT, sas10Δ2 and mpp10Δ10 embryos at 5dpf. The liver area was defined by immunostaining of Bhmt (green). Compared to the WT embryos, no abnormal apoptotic activities were observed in sas10Δ2 and mpp10Δ10 mutant embryos. DNaseI treated embryos were used as the positive control for apoptotic cells (red). At least 16 cryosections from three embryos were examined. Bar: 50μm. (B) Northern blot analysis of rRNA precursors using the 18SF (left panel) and 18S-B (right panel) probes in 5dpf-old WT, sas10Δ2 (sΔ2) and mpp10Δ10 (mΔ10) embryos. 28S: loading control.

Acknowledgments

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