ZFIN Image: Ye et al., 2019, Fig. 3

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Figure Caption

Fig. 3

The abundance of early PGCs promotes female development. a Representative images showing PGCs in wildtype and buc-overexpressed embryos at shield stage (PGCS was visualized by WISH). b Percentage of embryos with increased PGCs or normal number of PGCs in wildtype and buc-overexpressed embryos. c Representative images showing PGCs in wildtype and buc-overexpressed embryos at 1 dpf (PGCS was visualized using Tg(piwil1:egfp-UTRnanos3)^ihb327Tg). d The sex ratio in population of wildtype and buc-overexpressed embryos. e RT-qPCR showing that buc was overexpressed in Tg(kop:KalTA4-UTRnanos3,CMV:EGFP)^ihb8Tg/Tg(UAS:buc-UTRnanos3)^ihb120Tg double-transgenic fish (dual-Tg) and Tg(UAS:buc-UTRnanos3)^ihb120Tg. f The sex ratio in the populations of sibling control (Tg(kop:KalTA4-UTRnanos3,CMV:EGFP)^ihb8Tg and wildtype fish), Tg(UAS:buc-UTRnanos3)^ihb120Tg and double transgenics

Acknowledgments

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