Fig. 1

Fig. 1

 Vitamin D Analogs Promote Cardiomyocyte Proliferation in Zebrafish

(A) Cartoon schematic of in vivo chemical screen for regulators of CM proliferation using cmlc2:FUCCI zebrafish embryos. dpf, days post-fertilization.

(B) Maximum intensity projection (MIP) images of dissected cmlc2:FUCCI hearts. Veh, vehicle; v, ventricle; a, atrium. Scale bar, 50 μm.

(C) Quantification of FUCCI⁺ CMs after 24-h treatment. Mean ± SD, Mann-Whitney test and Student’s t test. ^∗∗∗∗p < 0.0001. n = 11–13.

(D) Quantification of FUCCI⁺ CMs after 24-h treatment. Mean ± SD, Mann-Whitney test and Student’s t test. n = 7–10.

(E) Quantification of adult ventricular CM proliferation indices. Mean ± SD, Mann-Whitney test and Student’s t test. ns, not significant; ^∗∗p < 0.01. n = 9–10.

(F) Representative images of PCNA/Mef2 staining quantified in (E). Boxed area is enlarged on the right. Arrowheads indicate PCNA⁺CMs. dpt, days post-treatment. Scale bar, 100 μm.

(G) Ventricular CM proliferation indices of vehicle- or Alfa-injected adult zebrafish. Mean ± SD, Mann-Whitney test and Student’s t test. n = 10–12.

(H) Representative images and quantification of ventricular PCNA/Mef2 staining. Mean ± SD, Mann-Whitney test. n = 9–10. Scale bar, 100 μm.