Fig. 5

Fig. 5

pcdh10b is up-regulated and genetic compensates inpcdh10a mutant embryos (A) Real-time PCR comparing wildtype embryos to pcdh10a-/- mutants, normalized to ef1a, * denotes P-Value< 0.05. (B-C) ISH probing for pcdh10bexpression in (B) wildtype and (C) pcdh10a-/- mutants. (D-I) Whole-mount lateral views of 72hpf embryos. Wildtype embryos with no Morpholino injected (D), 2 ng pcdh10bMorpholino injected (E), Standard control Morpholino injected (F). (G,H,I) Homozygous pcdh10a-/- mutants. With no Morpholino injected (G), 2 ng Morpholino injected (H), Standard control Morpholino injected (I). Insets are blown up images to highlight internal melanocytes (red arrows) or lack of internal melanocytes. (J) Mortality rate percent of wildtype embryos and mutant embryo injected with pcdh10bMorpholino. (K, L) Representative images of sibling embryos at 72hpf from the same cross. (K) pcdh10a-/- embryo that is wildtype for pcdh10b. (L) Embryos that are homozygous mutants for both pcdh10a and pcdh10b paralogs. Insets are blown up images to highlight internal melanocytes (red arrows). (M) Quantification of the percent of embryos with internal pigment cells., (N) Quantification of the number of melanocytes in the lateral stripe, **** denotes P-value of 0.0001. (M, N) wildtype n = 80, AB 1 ng pcdh10a MO n = 59, AB 2 ng pcdh10a MO n = 39, pcdh10a -/- 1 ng pcdh10b MO n = 113, pcdh10a -/-, 2 ng pcdh10b MO n = 22.