ZFIN Image: Tomar et al., 2019, Fig. 3

Image

Figure Caption

Fig. 3

Loss of MCU Limits _mCa²⁺ Uptake and Elevates Total Body Fat in Zebrafish

(A) Schematic of the generation of global MCU KO zebrafish using CRISPR/Cas9.

(B) Genotyping for MCU deletion.

(C) Bar graph showing MCU mRNA abundance in WT, MCU^+/−, and MCU^−/− zebrafish.

(D) Western blot for MCU expression. n = 3.

(E) Measurement of _mCa²⁺ uptake. n = 5–10.

(F) Quantification of ionomycin-induced peak _mCa²⁺ levels from (E). n = 5–10.

(G) Bar graph representing cellular ATP levels in cells isolated from MCU^+/+, MCU^+/−, and MCU^−/− zebrafish. n = 4.

(H) Adult WT and MCU^−/− zebrafish were homogenized and centrifuged. MCU^−/− zebrafish samples show a clear yellow color lipid layer on top of the protein lysate. n = 6.

(I) Adult WT and MCU^−/− zebrafish were stained with Lipid Green to monitor the distribution of lipids in the whole body. n = 3.

(J and K) Bar graphs represent the quantification of Lipid Green staining from the dorsal (J) or tail (K) fin. n = 3.

Statistical analysis: mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.

Figure Data

Phenotype details

Acknowledgments

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