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Fig. S1
Reverse transcriptase PCR (RT-PCR) and in situ RNA hybridization of spx1 and spx2 during early developmental stages in zebrafish. (A) RT-PCR of spx1 and spx2. cDNA from early developmental stages were used. β-actin was used as a control gene for RT-PCR. (B) In situ RNA hybridization using spx1 anti-sense RNA probe at 24 hpf. Lateral view of the brain and anterior is to the left. Arrowheads indicate the expression of spx1 mRNA in the hindbrain. (C) Transverse sections of the brain in (B) and dorsal is to the top. Arrowhead indicates the spx1-expressing cell in the hindbrain. Abbreviation: -ss, somite-stage. Scale bar: 100 μm in B and C.