Fig. S2

Fig. S2

Proportion of normal (blue), moderate (purple) and severe (red) phenotypes observed at 48 hpf after morpholino knockdown and mRNA rescue experiments. The number (n) of specimens analysed is indicated for each treatment. Treatments: control embryos injected with H₂O; control embryos injected with a control standard MO (1.6 mM); single morphants injected with either dlx5a or dlx6a translation-blocking MOs (0.8 mM); double morphants co-injected with dlx5a and dlx6a e2i2 splice-blocking MOs (0.4 mM each); double morphants co-injected with dlx5a and dlx6a translation-blocking MOs at two different concentrations (0.4 mM or 0.8 mM each); control embryos injected with GFP mRNA (200 ng/μl); embryos co-injected with dlx5a/6a translation-blocking MOs (0.8 mM each) and GFP mRNA (200 ng/μl) and embryos co-injected with dlx5a/6a translation-blocking MOs (0.8 mM each) and mutated dlx5a/dlx6a mRNAs (70 ng/μl each). Scale bar 100 μm.