ZFIN Image: Goudarzi et al., 2019, Fig. 5

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Figure Caption

Fig. 5

No non-coding function for squint 3’UTR.

(A) The position of untranslated regions (brown), coding region (green), putative Dorsal Localization Element- DLE (blue) and the gene deletion (red dashed line) in the squint genomic locus. Arrows flanking black dotted line mark the primer binding sites for qRT-PCR product. (B) In situ hybridization for squint at 8-cell stage on wild-type (18/20) and MZsquint^a175(17/17) embryos. (C) qRT-PCR for squint and eif4ebp1 on wild-type and MZsquint^a175 embryos at 1-cell stage. (D) Two representative MZsquint^a175 embryos. (E) MZsquint^a175 embryonic phenotype (N = 4 independent crosses, n = 360 embryos). The statistical significance of the observed changes was determined using t-test analysis and represented by star marks (ns, *, **, ***, and **** respectively mark p-values≥0.05,<0.05,<0.01,<0.001 and<0.0001).

Figure Data

Phenotype details

Acknowledgments

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