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Fig. 3
Expression of hdac4 and sox9a mRNA in the pharyngeal skeleton of wild-type embryos at 72 hpf, detected using mRNA in situ hybridization.
(A) Schematic of skeletal elements, lateral view for B–D, blue indicates cartilage. (B–D) expression of hdac4 and sox9a, arrows indicate expression in hyosymplectic, ceratohyal, and palatoquadrate cartilages. (E) Schematic of skeletal elements, ventro-lateral view for F–H. (F–H) expression of hdac4 and sox9a, arrows indicate expression in ceratohyal and hyosymplectic cartilages. (I) Schematic of skeletal elements, blue indicates cartilage, red indicates bone, ventral view for J–L. (J–L) expression of hdac4 and sox9a, arrows indicate expression in the posterior pharyngeal arches. Abbreviations: bb, basibranchial; ch, ceratohyal; hs, hyosymplectic; ih, interhyal; m, Meckel’s cartilage; op, opercle; pa3-7, posterior pharyngeal arches 3-7; pq, palatoquadrate. Scale bar = 50 microns.
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