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Fig. 5
Loss of Ntrk2b effects on serotonin levels and its markers at 3 dpf. (A) Total serotonin levels were reduced in the morphants at 3 dpf. (B) The three TPH transcripts were analyzed in the morphants by QRT-PCR: Ba - tph2, Bb - tph1a, Bc - tph1b. (B) (a) Only tph2 was significantly reduced in the morphant and rescued by mRNA overexpression. (B) (b,c) The levels for tph1a and tph1b were unchanged. (C) (a–c) ISH with tph2 reveals reduced levels in the morphants. (D) (a–c) Expression of serta was observed in the raphe and pretectal cluster. Levels were reduced in the morphants. The y-axis for the Q-RT PCR represents relative expression levels normalized to b-actin. (Bars represent Mean ± SEM, Student t-test *p-value < 0.05, one-way ANOVA, Kruskal-Wallis test *p = 0.025, Scale bar = 100 µm).