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Fig. S1
a, Zebrafish embryos were treated from tailbud stage until 48 hours post fertilization (hpf) with atorvastatin and water-soluble cholesterol. b, Live images of embryos treated either with vehicle (1% DMSO in egg water, Control), 10 μM atorvastatin or 10 μM atorvastatin together with 10 μM cholesterol. Arrows indicate pericardiac cavity, which is less dilated upon cholesterol reconstitution. Scale bar: 200 μm. c, Formation of pericardiac oedema is partly rescued after cholesterol supplementation. n=3 experiments. * p=0.0239, *** p=0.0003. One-way ANOVA with Sidak’s post-test. d, Heart rate significantly increases by co-administering cholesterol to atorvastatin-treated fish. n=3 experiments, each circle is one embryo.. ** p=0.0056, **** p<0.0001. Kruskal-Wallis test with Dunn’s multiple comparison test. Red line: median. Number of embryos given below graphs.