ZFIN Image: Maerz et al., 2019, Fig. 6

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Fig. 6

Cholesterol depletion alters phosphoinositide levels at the base of the cilium. a Confocal stacks of cilia showing Rab8-positive vesicles at the base of the cilium of human fibroblasts. Cilia were stained with an antibody against acetylated tubulin (acetTub, magenta). Vesicles are labelled in green (Rab8). Scale bar: 4 µm. b Distribution of Rab8 vesicle content at the base of the cilium between control treated and atorvastatin-treated human fibroblasts. Means ± SEM; n = 3 independent experiments. c Confocal stacks showing lack of Pi(4,5)P₂ (magenta) at the base of cilia, when cells were treated with atorvastatin during ciliogenesis. Cilia were labelled with an antibody against Arl13B (green). Scale bar: 4 µm. d Analysis of five independent experiments revealed a significant reduction of cells exhibiting Pi(4,5)P₂ at the base of the cilium. Each circle indicates the percentage of cells having Pi(4,5)P₂ at the base of the cilium in one experiment. Means ± SEM; n = 5 with 181–202 cilia in total; *p = 0.0269, two-tailed t-test with Welch’s correction. e SLOS patient-derived fibroblasts exhibit less Pi(4,5)P₂ at the base of the cilium. Means ± SEM; n = 4 with 154–156 cilia in total; **p = 0.0071, two-tailed t-test with Welch’s correction

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